Dr Evan Burkala
2001 - PhD Veterinary Studies, Murdoch University, Australia
1996 - BSc (Hons) Veterinary Biology, Murdoch University, Australia
1995 - BSc Biotechnology, Murdoch University, Australia
アメリカ在住。現在University of OklahomaのDepartment of Microbiology and Immunologyに勤務。分子ウイルス学と生物学での長年の経験を基に近年ではHIV免疫学と進化論を専門とする。多数の論文および研究助成金申請書のレビュー経験に加えて、癌生物学や免疫学を含む幅広い研究分野でのピアレビュー論文の発表実績を持つ。クローニング、PCR、リアルタイムPCR、組み換えタンパク質精製等の基本的なスキルに加え、ヘテロ2本鎖トラッキングアッセイや質量分析法など最先端の分子生物学的手法のスキルを持つ。免疫学のスキルとしては、フローサイトメトリー、ELISA、ウエスタン免疫ブロット法、ELISPOTなど。細胞培養では、初代細胞・細胞株培養、ウイルス培養と精製、トランスウェルマイグレーションアッセイ、共焦点顕微鏡法など。動物実験の分野では、免疫付与や免疫学的分析を目的としてマウス、ヒツジ、牛、ウサギを用いての研究経験がある。
Dr Burkala is currently employed at the University of Oklahoma in the Department of Microbiology and Immunology. Dr Burkala has many years of experience in molecular virology and biology, and has recently specialized in HIV immunology and evolution. He has peer-reviewed publications in a broad range of scientific areas including cancer biology and immunology in addition to reviewing many publications and grant applications. Dr Burkala’s technical skills include: cutting-edge molecular biological techniques such as heteroduplex tracking assays and mass spectrometry; common techniques like cloning, PCR, real-time PCR and recombinant protein purification; immunological techniques such as flow cytometry, ELISA, Western immunoblot and ELISPOT; cell culture techniques including primary and cell line culture, virus culture and purification, transwell migration assays and confocal microscopy; and live animal experiments including the use of mice, sheep, cattle, and rabbits for immunizations and immunological analyses.
